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Transformation Procedure 1. Take ice, put DH5a, wait to thaw 2. Aliquote 50 ul to prechilled 600 ul tubes 3. Add 1 ul DNA to tubes and wait for 30 min on ice. 4. Heat shock 42oC for 45 sec. 5. Incubate on ice for 2 min
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Document Date: 2017-09-15 13:58:59
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Transformation Procedure 1. Take ice, put DH5a, wait to thaw 2. Aliquote 50 ul to prechilled 600 ul tubes 3. Add 1 ul DNA to tubes and wait for 30 min on ice. 4. Heat shock 42oC for 45 sec. 5. Incubate on ice for 2 min