| Document Date: 2014-01-29 14:52:41
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City Berlin / Choi / Washington DC / Surrey / Burley / / Company European Molecular Biology Laboratory / FMC / Brown / Hershey / Elowitz & Stanislas Leibler / Fletcher S. P. / Macmillan Magazines Ltd / CRC Press / / Country United States / / / Facility Scale bar / Princeton University / European Molecular Biology Laboratory / / IndustryTerm arti®cial genetic network / intracellular networks / fresh media / designed networks / protein product / transcriptional repressor systems / minimal media / biotechnological applications / oscillating network / arti®cial network / coupled chemical reactions / synthetic network / repressilator network / simplest such networks / synthetic oscillatory network / numerical solution / biochemical networks / / Organization National Institute of Health / Cambridge Univ. / Humboldt Foundation / Princeton University / Princeton Univ. / / Person D'Ari / / Position damaged messenger / Walker / T.V.P. / Repressilator Reporter / messenger / reporter / / ProvinceOrState New Jersey / S. K. / M. B. / / PublishedMedium Molecular Biology / / Technology antibodies / gene expression / cloning / simulation / ATG / / URL www.nature.com / /
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letters to nature Western blotting Ribosomal complexes assembled and puri®ed as described above were TCA-precipitated. Proteins were resolved on 12% polyacrylamide gel, transferred to nitrocellulose membrane and probed